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Contribution of Viral Genomic Diversity to Oyster Susceptibility in the Pacific Oyster Mortality Syndrome ArchiMer
Delmotte, Jean; Chaparro, Cristian; Galinier, Richard; De Lorgeril, Julien; Petton, Bruno; Stenger, Pierre-louis; Vidal-dupiol, Jeremie; Destoumieux-garzon, Delphine; Gueguen, Yannick; Montagnani, Caroline; Escoubas, Jean Michel; Mitta, Guillaume.
Juvenile Pacific oysters (Crassostrea gigas) are subjected to recurrent episodes of mass mortalities that constitute a threat for the oyster industry. This mortality syndrome named “Pacific Oyster Mortality Syndrome” (POMS) is a polymicrobial disease whose pathogenesis is initiated by a primary infection by a variant of an Ostreid herpes virus named OsHV-1 μVar. The characterization of the OsHV-1 genome during different disease outbreaks occurring in different geographic areas has revealed the existence of a genomic diversity for OsHV-1 μVar. However, the biological significance of this diversity is still poorly understood. To go further in understanding the consequences of OsHV-1 diversity on POMS, we challenged five biparental families of oysters to two...
Tipo: Text Palavras-chave: Crassostrea gigas; Herpesvirus diversity; Genotype-genotype interactions; Oyster genetic background; Viral populations.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00643/75533/76414.pdf
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Oyster hemolymph is a complex and dynamic ecosystem hosting bacteria, protists and viruses ArchiMer
Dupont, Samuel; Lokmer, A.; Corre, E.; Auguet, Jean-christophe; Petton, Bruno; Toulza, E.; Montagnani, Caroline; Tanguy, G.; Pecqueur, D.; Salmeron, C.; Guillou, L.; Desnues, C.; La Scola, B.; Bou Khalil, J.; De Lorgeril, Julien; Mitta, Guillaume; Gueguen, Yannick; Escoubas, Jean Michel.
Background The impact of the microbiota on host fitness has so far mainly been demonstrated for the bacterial microbiome. We know much less about host-associated protist and viral communities, largely due to technical issues. However, all microorganisms within a microbiome potentially interact with each other as well as with the host and the environment, therefore likely affecting the host health. Results We set out to explore how environmental and host factors shape the composition and diversity of bacterial, protist and viral microbial communities in the Pacific oyster hemolymph, both in health and disease. To do so, five oyster families differing in susceptibility to the Pacific oyster mortality syndrome were reared in hatchery and transplanted into a...
Tipo: Text Palavras-chave: Oyster genetic background; Hemolymph microbiota dynamics; Early-life microbiota; Trans-kingdom interactions; Crassostrea gigas; Within-host ecosystem.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00627/73916/73254.pdf
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